經(jīng)典案例|普健哺乳動(dòng)物細胞表達系統之scfv表達

（1）Protein Name: protein L（一種scfv）

該蛋白不易表達，采用哺乳動(dòng)物細胞表達系統表達活性最佳，其他表達系統表達活性低。

（2）Expression vector: pATX2

（3）Expression host: HEK293F

總結：蛋白L（scfv）采用普健高表達pATX系列載體配合公司優(yōu)化高密度哺乳表達系統Xten293，成功表達，表達量高達31.2mg/L，純度>90%，經(jīng)檢驗，其具有較高活性。

MW. Molecular weight marker. Ø. Non-transfected (negative control).

The protein L expressed secretorily in HEK293F cells.

MW. Molecular weight marker. IN. Input.  FT. Flow through. W. Washes. E. Eluted fractions.

A. The purification test result. B. Final QC gel.

L-pATX2 was transfected with 80ml HEK293F cells. The culture medium and cells which were collected on the sixth day post transfection did purification test.

1L scale up purification

MW. Molecular weight marker. IN. Input.  FT. Flow through. W. Washes. E. Eluted fractions.

A. The purification test result. B. Final QC gel.

瞬時(shí)轉染表達

重組抗體表達

穩定細胞株構建

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